Barcoded Overhang Adapter Kit - 8A

Two sets of 8 barcoded overhang adapters for multiplexing SMRTbell libraries in applications such as multiplexing for microbial whole genome sequencing when using the Express 2.0 product family.

Product Details
The Barcoded Overhang Adapter Kit – 8A/8B are used in conjuntion with the SMRTbell Express Template Prep Kit 2.0 (PN 100-938-900) for multiplexed microbial multiplexing sequencing. The kits can be combined to perform up to 16-plex of pooled microbial genomes. For the Sequel System, we recommend pooling up to 30 MB of total microbial genomes per SMRT Cell to obtain highly contiguous microbial genome assemblies, and up to 40 MB for advanced users. For Sequel II and Sequel IIe Systems, up to 48 microbial genomes can be pooled together per SMRT Cell 8M using the 16 barcodes from this kit and/or supplementing with customer oligos from any oligo synthesis provider. The specific barcodes contained in this kit are as follows for the respective kits: 8A: bc1001, bc1002, bc1003, bc1008, bc1009, bc1010, bc1011, bc1012. 8B: bc1015, bc1016, bc1017, bc1018, bc1019, bc1020, bc1021, bc1022.

Each kit contains 8 barcoded overhang adapters with sufficient reagents to perform 6 ligation reactions for each barcode to support multiplexed microbial genome sequencing, or 12 reactions for amplicon sequencing.

Note: Compatible with all Sequel, Sequel II and Sequel IIe Systems