Estándar de referencia cuantitativo multiplex (formato FFPE) 50ng_µl, =400 ng_pieza

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Quantitative Multiplex Reference Standard (QMRS) portfolio covers multiple endogenous SNPs, insertions and deletions. The QMRS includes 11 mutations at 0.8-24.5% allelic frequency in genomic DNA, FFPE and Formalin-Compromised DNA format. The multiple formats of the QMRS allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. The FFPE format QMRS allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. With the Formalin-Compromised format, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate these highly degraded samples.

The QMRS standard is our most popular multiplex standard as it provides both a breadth of formats, but also a large range of allelic frequencies and onco-relevant mutants.

Technical Data

• Genes Covered BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA Cosmics 1,500+
• Fixation Method 10%
• Formalin Section Size 15µm or 20µm
• Cell Density 3 x 108 cells per block.
• Approx. 3.5 x 105 cells per section
• Expected DNA Yield > 400 ng DNA using Promega Maxwell LEV Plus Extraction kit
• Product information- please check in the Horizon webpage https://www.horizondiscovery.com