SMRTbell Express Template Prep Kit 2.0

Quickly construct SMRTbell libraries for all insert sizes from 250 bp to >50 kb in as little as 4 hours.

Product Details
The SMRTbell Express Template Prep Kit 2.0 (TPK 2.0) supports insert sizes from 250 bp to >50 kb. This kit employs a single-tube, addition-only strategy to minimize handling-induced DNA damage for library generation in as little as 4 hours, excluding size selection. We recommend this kit for most applications except for shotgun metagenomics (use TPK 1.0, PN: 100-259-100) or large-insert HiFi reads are required. To generate large-insert HiFi reads, the SMRTbell Enzyme Clean Up Kit (PN: 101-746-400) and Sequencing Primer v2 (PN: 101-847-900) are also needed which can be purchased together in the TPK 2.0 Bundle (PN: 101-843-100).

Each kit contains sufficient reagents to prepare either 18 standard reaction large-insert libraries, 18 Iso-Seq libraries, 48 multiplexed microbial genomes, or 96 reactions for amplicon sequencing.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.