Sequel II Binding Kit 2.0 and Internal Control Kit 1.0

Optimized reagents for binding SMRTbell template libraries with inserts >3 kb to a DNA polymerase for sequencing on the Sequel II and Sequel IIe Systems.

Product Details
The Sequel II Binding Kit 2.0 and Internal Control Kit 1.0 is a bundle kit of Sequel II Binding Kit 2.0 (PN: 101-789-500) and Sequel II DNA Internal Control Complex 1.0 (PN: 101-717-600). This bundle kit, and specifically Sequel II Binding Kit 2.0, should primarily be used with SMRTbell inserts >3 kb. The Sequel II Binding Kit 2.0 contains reagent supplies to bind prepared DNA template libraries to the Sequel II Polymerase 2.0 in preparation for sequencing on the Sequel II and Sequel IIe Systems. The result is a DNA polymerase/template complex. Sequel Polymerase 2.0 should be used only with Sequel Sequencing Kit 2.0. Sequel II DNA Internal Control Complex 1.0 contains a fixed template of 2 kb length, bound to Sequel II DNA Polymerase 1.0 for use as an internal sequencing control on the Sequel II and Sequel IIe Systems.

Reagent quantities support a minimum of 24 binding reactions and up to 70 SMRT Cell runs per binding reaction, depending on sample insert size, application, and concentration.

Notes: Sequel II and Sequel IIe Systems compatible only. Sequel II DNA Internal Control Complex 1.0 (PN: 101-717-600) also sold separately.