MasterMix PyroTaq Probe qPCR Plus 5x, 2 ml (500 rxn)

PyroTaq PROBE qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and PyroTaq HS (Hot Start DNA Polymerase) supplied in a proprietary reaction buffer that enables detection of low copy number targets. PyroTaq HS (Hot Start DNA Polymerase)is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Aplications: 

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Components:

• PyroTaq HS (Hot Start DNA Polymerase)
• 5x Probe qPCR buffer
• 15 mM MgCl2
• 1x PCR solution – 3 mM MgCl2
• dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
• No ROX dye
• BSA