MasterMix qPCR No-ROx PyroTaq EvaGreen 5x, 2 ml (500 rxn)

Compatibility:

• Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®
• Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
• Eppendorf: Mastercycler®: ep realplex2 & ep realplex4 

• llumina: The Eco™  
• Roche: LightCycler® 480




Composition:

• PyroTaq EvaGreen qPCR Master Mix (NO ROX) 5X
• 5x EvaGreen® qPCR buffer
• 12.5 mM MgCl2 1x PCR solution – 2.5 mM
• dNTPs
• EvaGreen® dye
• No ROX dye 


EvaGreen® Dye:

• EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR.
• Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I
• EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic.
• EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green