XL10-Gold Competent Cells, 10 x 100 ul
The XL10-Gold ultracompetent cells were designed for cloning large plasmids and ligated DNA with the highest transformation efficiency possible, while exhibiting faster growth and larger colonies. XL10-Gold Ultracompetent Cells possess the high transformation phenotype (Hte), which gives these cells an average transformation efficiency of ≥ 5 x 10^9 transformants/μg of supercoiled DNA Plasmid libraries constructed in this strain are more representative because XL10-Gold cells decrease the bias against large inserts.
- Engineered to transform large plasmids and ligated DNA more efficiently than other cell lines
- Includes Hte phenotype for 20-fold to 30-fold higher transformation efficiency of ligated DNA and better competent cell performance
- Chemically competent cells that allow efficient transformation of large DNA molecules, including expression vectors and genomic DNA
- Ideal for plasmid library construction by decreasing bias against larger plasmids and producing more colonies for a more representative library
- Transformation efficiency of ≥5 x 10^9 transformants/µg
Id. Nav | 200315 |
---|---|
Envase | 10 x 100 ul |
Aplicaciones | Clonaje |
Categoría de Producto | Células competentes |
Disponibilidad | Bajo pedido |
Especificaciones | Blue-White Screening Available: Yes Cell Strain: XL-10 Cell Type - Production Method: Chemically Competent Competent Cell Application: Cloning large or ligated DNA Difficult Cloning Type: Large DNA, Ligated DNA Generates Unmethylated DNA: No Resistance: Chloramphenicol. Tetracycline Transformation Efficiency - Qualitative: High Transformation Efficiency - Quantitative: ≥ 5 x 109 transformants/µg Volume: 10 x 0.1 mL |
Marca | AGILENT |
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