StemFit Basic 03

Stem cell medium for clinical research

StemFit® is a cell culture medium for clinical research into regenerative medicine,. This stem cell media is produced under strictly controlled conditions and is GMP compliant under Japansese guidlines. StemFit® Basic03 is completely free of animal and human components. All ingredients are recombinant.

Recommended by the nobel prize winner Shin’ya Yamanaka

“StemFit®, a newly developed Xf-medium, was the best medium for hESC and hiPSC culture with rLN511E8.” 

Feeder cells and Xeno-free culture medium

StemFit® medium was developed to produce a reliable and well-defined growth condition for stem cells. It contains only Xeno-free components and all necessary components for the culture of embryonic as well as induced Pluripotent Stem (iPS) cells. These important benefits lead to a reduction of variances and contaminations in the cultivation of stem cells, when using the StemFit® medium.

Very reproducible growth rate

The cultivation of stem cells using StemFit® results in a very reproducible growth rate.This enables a perfect planning of experiments. No more variation due to different starting conditions caused by the natural variation when culturing on feeder cells. Analysing the morphology of stem cells cultivated in StemFit® shows that the colony shape and size are very similar to the cells grown on feeder cells

Less media volume needed

Due to the high-quality components and the ideal concentration of nutrients, the required volume of StemFit® is lower than of conventional media. Additionally, reduced feeding steps during the week, creates a total volume difference between StemFit® and conventional media of more than 50%

Weekend-free feeding

The maintenance of stem cells is very complicated and labor intense and the feeding step during the weekends is very tedious. StemFit® allows a feeding-free weekend, by using the recommended weekly workflow shown below 

Start from a single cell

StemFit® enables a superior colony forming efficiency from a single cell clone, which minimize the effects of stress and result in reliable cells for downstream applications. Furthermore, with StemFit® you can easily determine the efficiency of your cell production and duplicate individual clones.

Easy transition from feeder cells

Majority of the stem cells are still maintained in feeder-cell dependent cultivation. The transition from feeder-cells to nonfeeder-cells system could not be easier: Switch the medium twice before passaging the cells and continue cultivating them in StemFit®. After 2-3 passages the feeder cells will be completely diluted out and a pure stem cell culture will be obtained 

Compatible with many matrices

StemFit® has been tested on many different matrices. As shown on Fig. 6., the fold expansion rate of cells cultured in StemFit® is much higher when compared to medium A from the market leading competitor.

Consistent gene expression profile

The cultivation of stem-cells is very stressful for the cells. Every passage and growth period could therefore introduce unwanted changes in the genome expression profile. Hence, the CGI Catapult Institute in London investigated the genomic profile of StemFit® after 1 passage, 3 and 5 passages and compared it to 4 commercially available media. The most consistent gene expression was obtained using StemFit®.

Less production of lactate

The production of lactate is the result of hypoxic stress. The consequences can be changes in the genome expression profile or lead to unwanted differentiation of the stem cells. The CGI Catapult Institute in London showed that there is considerably less lactate production when the cells are grown in StemFit®