RNase Free DNase I, 5000U

DNase I, Rnase Free from bovine pancreas catalyzes the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.

Features

• Produces no noticeable RNA degradation
• One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume.
• Incubation of 100-fold molar excess DNase I with RNA for one hour at 37°C produces no noticeable RNA degradation upon analysis by denaturing polyacrylamide gel electrophoresis.
• Reaction conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
• Storage Buffer: 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)

Specifications

• Concentration: 10 U/µL
• Incubation Time: Incubate 30 minutes at 37 °C
• Physical Form: Premixed
• Reaction Conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
• Storage Buffer: 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)
• Substrate Type: RNA
• Unit Definition: One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25 µL reaction volume.