RNase Block, 4,000U

RNase Block Ribonuclease Inhibitor is a 50-kDa protein that specifically inhibits common eukaryotic RNases including RNase A, RNase B, and RNase C. It is a recombinant ribonuclease inhibitor derived by a molecular cloning technology that inactivates RNases by binding noncovalently to the RNase molecule. Only 25 to 40 U is required for a standard transcription reaction Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts with 40 U of RNase Block Ribonuclease Inhibitor for 1 hour at 37°C and by checking for the absence of degradation. Applications: RNA transcription, In vitro RNA translation, RNA capping, and Any application where eukaryotic RNase contamination is a potential problem RNase Block does not inhibit RNase H, RNase T1, T7, T3, or SP6 RNA polymerases, Taq DNA polymerase, AMV or MMLV reverse transcriptases Concentration: 40 U/µL Storage Buffer: 20 mM HEPES-KOH (pH 7.6), 50 mM KCI, 8 mM DTT and 50 % (v/v) glycerol Unit Definition: One unit will inhibit 50 % of the activity of 5 ng of RNase A.