RNace-It Ribonuclease Cocktail

RNace-IT ribonuclease cocktail is a mixture of two RNases: RNase A and a nonspecific RNase from Aspergillus oryzae known as RNase T1. The combined action of these ribonucleases results in the degradation of RNA into small oligoribonucleotides. Reaction Conditions: active in any neutral buffer (pH 6.0-9.0) or water with sodium chloride concentrations ˂ 100 mM Storage Buffer: 10 mM Tris-HCl (pH 7.5), 15 mM NaCl Unit Definition: 20 units will degrade the RNA in a 1.5 ml plasmid preparation