With the effectiveness of 3D spheroids in many areas of research including anti-cancer drug screening and in vitro tumor studies, the need for better methods to produce replicate spheroids of uniform size in mass quantities has emerged. Corning Elplasia plates address this need by enabling researchers to generate a high density of spheroids in a scaffold-free model. Use Corning Elplasia plates to generate, culture, and analyze your spheroids all in a standard plate footprint.
Corning Elplasia plates are available in multiple formats, two well geometry types, and two surface coatings. Corning Elplasia Round Bottom Plates Corning Elplasia round bottom plates are optimal for bulk spheroid formation, assay, expansion, and collection. Round bottom plates are available in 6-, 24-, and 96-well formats and all feature Corning Ultra-Low Attachment (ULA) surface. Corning ULA surface is a proprietary, animal-free, covalently bonded hydrogel surface that is hydrophilic and neutrally charged.
The ULA surface promotes the formation and easy harvesting of anchorage-dependent scaffoldfree spheroids. Corning Elplasia Square Bottom Type Plates Corning Elplasia square bottom type plates feature a surface with optical qualities suited for image analysis, making them an ideal solution for clonal selection and high magnification imaging of very small clusters. Square bottom plates are plasma-treated for self-coating and are available in 6-, 24-, 96-, and 384-well formats.
Benefits
Corning® Elplasia® plates are compatible with many cell types and may be used across many applications including:
Available in two well geometry and surface coating options: round bottom type featuring Ultra Low Attachment (ULA) surface and square bottom type featuring a plasma-treated surface for self-coating
Multiple format options: 6-, 24-, 96-, and 384-well
Black opaque sidewalls that reduce well-to-well “cross-talk”
Square well plates feature a surface with optical qualities suited for image analysis
Gamma irradiated
One-year shelf life
Key Benefits
Create uniform spheroid formation at large volumes with a simple and easy to use “plug and play” protocol
No rinsing required prior to seeding
Generate and culture spheroids in one plate – for up to 21 or more days
Highly reproducible bulk spheroid formation across microcavity wells – from 79 to 15,000+ spheroids per well
Culture a high density of spheroids in one plate under one culture condition Increased signal per well without an increase in spheroid size
High density format also generates increased data points, enabling image analysis of multiple spheroids vs. one spheroid per well
Suitable for fluorescent/luminescent assays
The square well plates are ideal for clonal selection and high magnification imaging of very small clusters
