CloneticsHuman Skeletal Muscle Cells (SkMC) and Myoblasts (HSMM) are isolated from normal donors. HSMMs are isolated from the upper arm or leg muscle tissue, and SkMCs are isolated from gestational tissue usually from the quadriceps or psoas tissue. Both are sold in second passage. HSMMs can be differentiated to form myotubes in culture.
CloneticsHSMMs are guaranteed through 10 population doublings and to differentiate to form multinucleated myotubes in culture. SkMCs are guaranteed through 15 population doublings and to stain positive for desmin. Both cell types test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
Recommended medium: HSMMs require SkGM-2 BulletKitand SkMCs require SkGMBulletKit
Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
In addition to cryopreserved cell ampules Lonza also offers viable cells: Clonetics™ Proliferating Cells are available in a variety of convenient flask and plate formats.
Proliferating cells are delivered Tuesday-Thursday the week after initial plating. Confluency at the time of shipment varies by cell type. A new Clonetics™ Cryovial is used for each new batch using only Clonetics™ Media and Reagents. This procedure ensures viable cells of Benefits of Clonetics™ Proliferating Cells
Save valuable time and materials by using viable cells: No need for expensive thawing and culture expansion. Just order the number of vessels you need.
Proof your results: Validate your cell line experiments with a reliable ready to use primary cell culture
Characteristics of Clonetics™ Proliferating Cells
More than 100 cell types available – normal and diseased
Variety of flasks and plates
Ready-to-assay in plate formats
CoA with donor characteristics
40+ years of experience in primary cells with most publications