Medio de congelación, Bambanker, 5 botellas de 20ml
Long-term storage of cultured cells
Cryopreservation of mammalian cells is extremely valuable and common in biological research. Fear of losing a cell line to contamination or incubator failure is frequently the impetus for making archival storage of cells a high priority after receiving or generating a new cell line. Once transferred from growth media to freezing medium, the cells are usually frozen at a controlled rate and stored in liquid nitrogen vapor or at -130°C in a mechanical deep freeze. Although freezing a cell line is a commonly performed procedure, problems arise when suitable freezers are not available, or unwanted variables are introduced by the presence of serum, extra-wash or complicated freezing algorithms.
Save time while saving your cells
The cell freezing media BambankerTM permits cryopreservation of cells at -80°C (or in liquid nitrogen), obviating the need for an additional and expensive ultralow freezer and avoiding time consuming and complicated controlled freezing protocols. Simply (1) harvest cells, (2) aspirate medium, (3) resuspend in BambankerTM (4) transfer to a cryovial and (5) store at -80°C. No programmed or sequential freezing is required! BambankerTM is a serum-free cryopreservation medium that is delivered ready-to-use and can be kept in the refrigerator for up to two years. Convenient 20 ml bottles are available, making BambankerTM freezing medium ideal for use by individual members of your lab. Today this innovative cell freezing medium BambankerTM is the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.
Serum adds variation to long-term storage
All BambankerTM products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of BambankerTM is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.
Universal – even for preservation of stem cells
All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of even sensitive cells is much higher compared to regular media. For example the JCRB cell bank stores over 1,400 different cell lines with great success with BambankerTM.
Id. Nav | BB02 |
---|---|
Envase | 5 x 20 ml |
Aplicaciones | Bioprocesos, Preservación |
Categoría de Producto | Medios de congelación |
Temperatura Envío | Refrigerado 4º |
Temperatura Almacenaje | Refrigerado 4º |
Disponibilidad | Bajo pedido |
Capacidad/Volumen | 5 x 20 ml |
Ref Dutscher | 523303 |
Caracteristicas |
|
Marca | NIPPON GENETICS |
Recomendaciones
-
FastGene Restriction Enzyme, Enzima de restricción EcoR I (20000 U)50,78 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Hga I (100 U)50,78 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Hph I (1000 U)47,15 € sin ivaPara ver tus precios y comprar inicia sesión
-
Mix de ligación de ADN FastGene Kickspeed 2x (50 rxns)81,84 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Mun I (300 U)28,58 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Nru I (1000 U)42,17 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Pvu I (500 U)50,78 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Sca I (1000 U)50,78 € sin ivaPara ver tus precios y comprar inicia sesión
-
FastGene Restriction Enzyme, Enzima de restricción Sse9 I (500 U)50,61 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
FastGene Restriction Enzyme, Enzima de restricción Taq I (4000 U)42,17 € sin ivaPara ver tus precios y comprar inicia sesión
-
Filtro de cámara de cultivo celular, FastGene, 4 unidades53,94 € sin ivaPara ver tus precios y comprar inicia sesión
-
Medio de congelación Bambanker HRM (con albúmina de suero bovino) para células iPS y ES, 120 ml494,76 € sin ivaPara ver tus precios y comprar inicia sesión
-
Set grande de preparación de geles para ADN_ARN para Mupid-One, 1set para 2plus, resistente al calor, 1 unidad236,22 € sin ivaPara ver tus precios y comprar inicia sesión
-
Criotubos de 1 ml de rosca interna estériles, 500 Uds.267,84 € sin ivaPara ver tus precios y comprar inicia sesión
-
Kit de extracción de ADN, Releasy Advance, 50 reacciones 1,5 ml76,26 € sin ivaPara ver tus precios y comprar inicia sesión
-
Sistema de electroforesis de proteínas FastGene PAGE1.581,00 € sin ivaPara ver tus precios y comprar inicia sesión
-
MasterMix Universal para qPCR ICGreen con fluoresceína, FastGene, 500 reacciones, 1 tubo258,39 € sin ivaPara ver tus precios y comprar inicia sesión
-
Transcriptasa reversa FastGene Scriptase básica para RT-PCR, 20000 unidades, 100 reacciones137,40 € sin ivaPara ver tus precios y comprar inicia sesión
-
Marcador de peso molecular de ADN de 100bp, 1 tubo52,08 € sin ivaPara ver tus precios y comprar inicia sesión