MasterMix qPCR PyroTaq EvaGreen Supermix 5x, 5000 rxns

• Adenovirus Precursor pVII Protein Stability Is Regulated By Its Propeptide Sequence
• Inturi R, Thaduri S, Punga T
• PloS ONE 2013: e80617

Benefits:
- Highly specific and reproducible real time PCR
- Excellent efficiency in case of low copy number targets
- UNG treatment capability due to dNTP blend of dUTP/dTTP
- Superior performance with long (up to 500 bp) and GC-rich templates
- Blue visualisation dye for easy pipetting
 
Mix Compatibility:
- Appilied BioSystems: QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM
- Stratagene: MX3000PTM , MX3005PTM
- Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®
- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4 

- llumina: The Eco™  
- Roche: LightCycler® 480



Mix Composition:
Pyrotaq Evagreen qPCR Supermix - 250rxn
- 5x EvaGreen® qPCR buffer
- 12.5 mM MgCl2 1x PCR solution – 2.5 mM
- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- EvaGreen® dye
- Internal reference based on ROX dye 

- GC-rich Enhancer
- Blue visualisation dye

 
EvaGreen® Dye:
EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
 
ROX Dye:
ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.