Librería Silenciamiento Génico -TRC Lentiviral Genome shRNA Library- Human

Disponible
The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library to enable the scientific community to use RNAi to determine the function of human and mouse genes.
Id. Nav | RHS4012 |
---|---|
Envase | 1 x glycerol stock |
Aplicaciones | Silenciamiento génico |
Categoría de Producto | Librerías shRNA |
Subcategoría de Producto | TRC |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Humano |
Ref Dutscher | 77RHS4012 |
Especificaciones | Libraries available for applications, such as high throughput screening Cloned into lentiviral vectors Amenable to in vitro and in vivo applications, such as the creation of knockdown cell lines Lentiviral vector enables transduction of primary and non-dividing cells Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html TRC shRNA library details shRNA design and cloning: The TRC shRNA constructs were designed using an siRNA rules based algorithm consisting of sequence, specificity, and position scoring for optimal hairpins. The hairpin consists of a 21-base stem and a 6-base loop. The hairpins were cloned into the pLKO1 vector and sequence verified. Multiple (4 to 5) constructs were created for each target gene. The pLKO1 lentiviral vector enables efficient transduction of primary and non-dividing cells, such as neuronal cells, making it easy to perform RNAi studies in these hard to transfect cell lines. Stable selection is also possible using the puromycin selectable marker. Viral packaging: Replication-incompetent viral particles can be efficiently produced using lentiviral packaging plasmids co-transfected in 293T packaging cells. The Trans-Lentiviral packaging system, which offers maximum biosafety and high titers, is recommended. Controls Available controls include the pLKO.1 empty vector as a negative control and an eGFP shRNA as a positive control. |
Caracteristicas | Simple hairpin shRNAs in the pLKO.1 lentiviral vector designed by The RNAi Consortium (TRC) |
Marca | HORIZON |
Recomendaciones
-
Librería Silenciamiento Génico -SMARTvector Inducible Protease Lentiviral shRNA Pooled Library hEF1a, GFP - Mouse6.721,24 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD hCMV-TurboGFP Control Particles - Human814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Edit-R Human All-in-one Set of 3 Lentiviral sgRNA (200 µL, 10^7 TU_mL)2.427,77 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mEF1a-None Glycerol 7452,77 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -siGENOME siRNA Library - Ubiquitin Conjugation Subset 2 - SMARTpool - Mouse2.965,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Membrane Trafficking Lentiviral shRNA Pooled Library mCMV,GFP - Mouse5.600,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -CCSB Human ORFeome Collection (Release 3)6.746,18 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Expresión Génica -Yeast W303 MATalpha Strain (glycerol stock)114,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control mEF1a-TurboRFP UHT 32.814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -Accell Cyclophilin B Pool - Rat1.018,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenc. Génico SMARTvector Inducible Cell Cycle Regulation Lentiviral shRNA Pooled Library mCMV-GFP, Human6.721,24 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus siRNA Set of 4 - Mouse855,42 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -shMIMIC Lentiviral microRNA Pooled Library - Human-Mouse Conserved mCMV3.947,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Edición Génica -Edit-R crRNA Library - Phosphatases - Set of 4 - Mouse8.940,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mEF1a-TurboRFP Control Particles 6814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -siGENOME siRNA GAPD Control siRNA -Human170,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector GPCR Lentiviral shRNA Pooled Library hCMV-RFP - Human5.600,65 € sin ivaPara ver tus precios y comprar inicia sesión