Reliable Probe qPCR Clara™ Probe Mix is engineered to enable qPCRs with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan®, Scorpions® and molecular beacons. Powered by our unique hot start Taq DNA polymerase the qPCR mix is suitable for DNA detection for 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit. Although for routine RNA quantification we recommend 1-step protocols with Clara™ Probe 1-Step Mix for a more streamlined workflow.
Clara™ Probe Mix can be used for all types of probe-based qPCR applications, including gene expression analysis, SNP/allele detection, genotyping and allelic discrimination studies, species abundance quantification, and can offers high efficiency in both single and multiplex assays. Making this mix highly versatile and ideal for in-vitro diagnostic kit development as well as basic research.
The mix is also suitable for melt-curve analysis (with hybridisation probes only) and is available without passive reference dye (No-Rox), or with reverence dye as Lo-ROX, Hi-Rox and separate-ROX formulations. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.
