Kit fluorimétrico para detección de Furina SensoLyte® Rh110

The SensoLyte® Rh110 Furin Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of furin activity using a fluorogenic substrate.

• High sensitivity fluorimetric detection of furin activity (0.02pg/uL of furin)
• Compatible with HTS for drug discovery applications
• One-step homogeneous mix-and-read format
• Competetively priced
• Custom services available
• Expert Assay Support

Furin, is a proprotein convertase expressed in all mammalian cell types, and processes a variety of precursor molecules into their active forms, including hormones, enzymes, growth factors, receptors, plasma proteins, bacterial toxins, viral envelope proteins, etc. Furin plays an essential role in embryogenesis and homeostasis, as well as in various diseases such as Alzheimer's disease, cancer, anthrax and Ebola fever. Furin has specifically been identified as an anti-viral drug target for HIV or HBV infections. 

Upon cleavage by furin, the substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm.

The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The detection limit can reach as low as 0.02 ng/mL.

Storage and Handling

• Store all kit components at -20°C, except for Component C.
• Store Component C at -80°C.
• Protect Components A and B from light and moisture.
• Component D can be stored at room temperature for convenience.