Kit,DNA Extraction

The DNA Extraction Kit provides a simple, nontoxic method for efficiently isolating high-molecular-weight DNA from tissue, whole blood and cultured cells. Depending on the starting material, the entire extraction takes only two to three hours to complete and does not require phenol or chloroform. DNA isolated with the DNA Extraction Kit is free from contaminants and may be used directly for restriction digests, cloning, Southern blotting, PCR amplification, and other DNA analysis techniques.

The DNA Extraction Kit1 is a modification of a procedure based on separating contaminating protein from DNA by salt precipitation.2 The procedure involves digestion of cellular proteins, subsequent removal of the proteins by “salting out” using standard sodium chloride, precipitation of the DNA with ethanol and resuspension in the buffer of choice. The number of samples that may be processed simultaneously using this technique is limited only by the centrifuge space available.