Kit Diagnóstico ELISA, GFLV, 96 ensayos

GFLV is a grapevine pathogen and is spread over medium and long distances in infected propagative material and soil, and between plants in the field by dorylamoid nematodes. Fanleaf disease is characterized by malformations of leaves and canes, and by various types of chlorotic discolourations of the foliage. Bunches are reduced in number and size, ripen irregularly and show shot berries and poor berry setting. Yellow mosaic is typically characterized by various patterns of brilliant chrome-yellow discolourations of leaves and shoots. Yellowing is most prominent in spring, fading away as the season progresses (heat masking). Crop losses range from moderate (5-10%) to very high (up to 90% or more) according to the virulence of the virus strain and varietal susceptibility. Fruit quality is also affected by a decrease in sugar content and titratable acidity. American rootstocks suffer a decrease of pruning wood up to 50% and show lower rooting ability of cuttings and graft take.

Specificity and sampling instruction

The DAS-ELISA reagent consists of different antibodies. The antibodies were made against isolates of GFLV from Central Europe (Colmar, France) and Canada (Vineland Research Station, Ontario). The coating antibodies are polyclonal, the AP-conjugated antibodies are monoclonal (P. Ellis, personal communication). For testing grapevine, a special extraction buffer «Grapevine» (Art. No. 110123) is used at a ratio of 1:10 (w/v); for other plants, the extraction buffer «General» (Art. No. 110120) is used at a ratio of 1:20 (w/v). Young leaves or sprouting buds and juicy bark are good tissue sources for detecting GFLV in grapevine.

The product was developed in cooperation with Phyto Diagnostics Company Limited, North Saanich, BC, Canada.