Kit Diagnóstico ELISA, GFkV, 960 ensayos

GFkV is a graft-transmissible virus that causes symptoms of disease in V. rupestris. Other Vitis species can be infected but remain asymptomatic. In infected V. rupestris, symptoms include localized clearings (flecks) in the veinlets of young leaves. In older leaves, the symptoms diffuse into a mosaic pattern and the leaves wrinkle and curl upward. Symptoms persist during mild weather and disappear with the onset of hot temperatures.

Specificity and sampling instruction

The antibodies were made against an isolate of Rupestris «St. Georges» grapevine (4) and specifically detect GFkV in DAS-ELISA. Similar to the grapevine leafroll associated closteroviruses, mature leaves and bark (phloem) scrapings are good tissue sources for testing. For testing grapevine, a special extraction buffer «Grapevine» (Art. No. 110123) is used at a ratio of 1:10 (w/v); for other plants, the extraction buffer «General» (Art. No. 110120) is used at a ratio of 1:20 (w/v). The product was developed in cooperation with Agroscope, the Swiss centre of excellence for research in the agriculture and food sector.

Information on the antibodies

Coating IgG: polyclonal; conjugate: monoclonal