BIOREBA has three reagents available for the detection of different BYDV strains by DAS-ELISA (1).
They were made against virus strains similar to the main two groups identified in the UK (Type B and Type F) and to a Mexican isolate of BYDV-RPV, now called cereal yellow dwarf virus-RPV (CYDV-RPV).
Studies in Switzerland showed that all BYDV isolates present could be detected with our ELISA reagents (2). For ELISA, stalks and young leaves are good tissue sources (4).
These products have been developed in cooperation with the Swiss Federal Resarch Station for Plant Production of Changins (RAC), CH-1260 Nyon, Switzerland.
Barley yellow dwarf virus-type B (BYDV-B) (PAV-like) This reagents were made against a BYDV strain which is efficiently transmitted by both Rhopalosiphum padi and Sitobion (Macrosiphum avenae) and is common in Western Europe (2, 4). Serologically, this strain is closely related to BYDV «Type B» (5) and is similar to BYDV-PAV.
The reagents react in DAS-ELISA similarly with the homologous and the B-strain, whereas only to a limited extend with «Type F» (5). Stalks and young leaves are good tissue sources for the ELISA (4). Barley yellow dwarf virus-type F (BYDV-F) (MAV-like) These reagents were made against the BYDV «Type F» (5) (a MAV-like isolate), specifically transmitted by Sitobion (Macrosiphum avenae).
The reagents also cross react to a certain extent (ca. 25 %) with BYDV «B» isolates (5) transmitted non-specifically by Rhopalosiphum padi , S. avenae and some other cereal aphids (4). Stalks and young leaves are good tissue sources for the ELISA (4). Barley yellow dwarf virus-RPV (BYDV-RPV), now called cereal yellow dwarf virus-RPV (CYDV-RPV) The antibodies were made against a Mexican isolate BYDV-RPV (3), efficiently transmitted by Rhopalosiphum padi (6).
The reagents react in DAS-ELISA specifically with BYDV-RPV. Cross reactions with «Type B» and «Type F» (5) are neglectable. Stalks and young leaves are good tissue sources for the ELISA (4).
