Kit de transfección de células primarias para Nucleofector 4D-LV P3, Kit L

The P3 Primary Cell 4D-NucleofectorTM LV Kit L is suited for larger scale transfection of primary cells in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the 1 mL NucleocuvetteTM Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.

This kit has been determined to be optimal for transfecting following primary cells and many others that use Solution P3 as optimal NucleofectorTM Solution:

• B cell (human)
• CD34+ cell (human)
• DC (human)
• ES (human), H9
• Macrophage (human)
• Monocyte (human)
• T cell (human), stim.
• T cell (mouse), unstim.

*Note: From the five primary cell solutions (P1 – P5) that come with the 4D-NucleofectorTM System only P3 is available for the large-scale formats so far. Solution P3 is suited for most immune and stem cells (e.g. T cells, dendritic cells, CD34 hematopoietic stem cells). For other solutions please contact our Scientific Support.

Benefits

• Closed Nucleofection of up to 108 primary cells
• Conditions transferable from existing smaller scale protocols
• Simple manual filling via sterile injection port
• Transfection within seconds

Applications

• For large scale transfection of up to 1 x 108 cells
• Ex-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)
• Generation of large numbers of transiently modified primary cells for cell-based assays