Kit de transfección básico de células primarias fibroblastos de mamifero para Nucleofector 2b, 1 kit 25 reacciones

In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector^TM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector^TM 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector^TM Programs is tested in combination with the Basic Nucleofector^TM Solution for fibroblasts.

The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.

Benefits

• Optimization can be performed within 1 experiment 
• Detailed protocols guiding through the optimization procedure 
• Transfection efficiencies up to 90%
• Viabilities up to 98%

Applications

• Kit suited for fibroblasts from different mammalian species and various organs 
• Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.