Kit de cualificación operativa para FEMTO Pulse, 1 kit

Dramatically simplify life in your lab with an automated workflow that brings you the best in productivity and precision. Take a few minutes to set Operational Qualification Kit up and FEMTO Pulse will automate capillary electrophoresis — giving you the power to optimize runs for speed or resolution.

Innovative design features ensure you get gold-standard data, while the one-step setup reduces the risk of handling errors creeping into your results, as it can with manual agarose and lab-on-a-chip methodologies.

Capillaries are auto-flushed between runs to eliminate cross contamination and further underline data integrity. When it comes time to view results, our software processes the data and visualizes it in your preferred format. All you need to do is choose the kit for your application, set the instrument and load your samples — from 12 to 288 at once.

Technology The Fragment Analyzer Automated CE System allows to achieve the perfect balance of speed and resolution by altering capillary length. Longer arrays can take resolution down to 3 bp for fragments under 300 bp in length. Shorter arrays will get through runs in as little as 15 minutes for certain applications. The inlet side of the capillary array is arranged in an 8 x 12 format, with electrodes next to each capillary.

This allows direct injection via vacuum or voltage from standard 96-well plates. The capillaries are aligned in parallel in the detection window, which is positioned between the LED light source and the detector assembly. The outlets of the capillaries are bundled to a common reservoir, where they are connected to a ground. The capillary arrays are modular and can be exchanged in less than five minutes. Room temperature air is circulated around the capillaries to modulate the temperature during CE separation. The optical platform consists of an LED light source (470 nm excitation), camera lens and CCD detector.

Fluorescent light from the LED passes through the array window, exciting the intercalating fluorescent dye in the gel matrices. This is captured by the camera lens and spatially imaged onto the CCD detector. Imaging is constant, so you get real time, on-line detection of separations. The power supply provides appropriate voltage for electrokinetic sample injection and electrophoresis.

The recommended operating limits are ±16 kV, 3 mA total current (30 µA per capillary). A syringe pump provides controlled pressure or vacuum to the outlet reservoir of the capillary array. Vacuum injection can be especially useful for eliminating the effects of salt on nucleic acid uptake. Automation Built-in software automates capillary conditioning, sample injection, electrophoresis separation and data processing. You can visualize results in your preferred format and share reports easily.