Cryopreserved Human Hepatocytes
Every lot of human cryopreserved hepatocytes is characterized for post-thaw viability and yield, plateability (adherence to a collagen-coated surface), and some combination of Phase I and Phase II drug metabolizing enzyme activities, inducibility, and uptake and efflux transporter activity—documented in a certificate of analysis. We have a wide selection of lots that can be used for a variety of applications, including, but not limited to:
Drug metabolizing enzyme activity (stability, clearance, species comparison, metabolite profiling and ID)
Suspension drug transporter uptake
Time-dependent metabolism (plated assay for low-clearance compounds)
CYP450 Induction (mRNA and activity)
Plated drug transporter assays (uptake and efflux)
Toxicity assays
Request TRL's cryopreserved hepatocyte inventory.
All suspension qualified human cryopreserved hepatocytes are characterized for CYP450 and Phase II drug-metabolizing enzyme activity. Both single donor and pooled human cryopreserved hepatocytes can be used for a variety of applications including metabolic stability, intrinsic clearance, metabolite ID, metabolite profiling, species comparison studies, and uptake transporter studies.
