Triangle Research Laboratories (TRL), now part of Lonza, provides fresh animal hepatocytes from a variety of species and strains. Fresh animal hepatocytes can be used for a variety of in vitro applications, including, but not limited to; metabolism, drug-drug interaction, drug transporter activity, and toxicity of drug candidates. Each lot of fresh hepatocytes is tested before release to ensure that hepatocytes are high viability. Our fresh hepatocytes in suspension are at least 80% viable and plated hepatocyte monolayers are at least 85% confluent.
TRL schedules fresh animal hepatocyte isolations on every Monday throughout the calendar year. Register for our FreshAlerts email list to receive weekly notifications about upcoming isolations. You may also visit our isolation calendar to plan your order around our schedule. Our planned isolations typically include the following species/strains of animals:
Rat (Sprague-Dawley, Wistar, Wistar Hannover)
Mouse (CD-1, C57Bl/6)
Dog (Beagle)
Monkey (Cynomolgus)
Cryopreserved Animal Hepatocytes
Every lot of animal cryopreserved hepatocytes is characterized for viability, plateability (adherence to a collagen-coated surface), and general Phase I and Phase II drug metabolizing enzyme activities—documented in a certificate of analysis.
Cryopreserved Animal Hepatocytes for Suspension Applications
All suspension qualified animal cryopreserved hepatocytes have post-thaw viabilities over 80%. These lots are also characterized for general Phase I and Phase II drug-metabolizing enzyme activity.
Cryopreserved Animal Hepatocytes for Plated Applications
All plateable cryopreserved animal hepatocytes have post-thaw viabilities over 80%. Each lot can also be plated in a collagen-coated plate or similar. These lots are guaranteed to maintain a healthy monolayer (>85% confluency) for a minimum of 3 days in culture. These lots are also characterized for general Phase I and Phase II drug-metabolizing enzyme activity.
