FITC-labelled C-rich telomere probe

The sequence of the FITC-labeled C-rich telomere probe is constituted of CCCTAA repeats and is 5' labeled by the FITC fluorescent Dye. The sequence is specific for both human and mouse

In principle, fluorescence in situ hybridisation (FISH) should be able to provide information on telomere length of individual chromosomes. However the efficiency of DNA/RNA oligonucleotide hybridizations for telomeric repeats investigation is not sufficient. In comparison the use of PNA stabilizes PNA-DNA/RNA duplexes. When used with PNA probes, PNA-DNA hybridization is much faster within a couple of hours and results in significantly reduced background noise due to the high signal to background noise ratio.

Specifications

• Packaging. Kits components: 1 x 2ml tube containing 5 nmol of lyophilised probe
• Chemistry. Sequence one letter code: repeats of CCCTAA
• Properties. Absorbance (nm): 490. Emission (nm): 525
• Modification Position: 5'
• Conjugation type: Fluorescent dyes
• Modification Name: FITC
• Conjugation: Conjugated

Storage & stability

• Form: Dried
• Resuspension condition: Please refer to the protocol

Activity

• Detection Method: Probe
• Usage: Research use

Codes
Code Nacres: NA.51