Most CloneticsPrimary Cells are also available as proliferating cells in either flasks or plates, or as pellets in RNALater.
CloneticsHuman Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or verticle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2.
CloneticsNHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. CloneticsAoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
Recommended media: FGM-3 BulletKitfor NHCF-A and -V and SCGMBulletKitfor AoAF
In addition to cryopreserved cell ampules Lonza also offers viable cells: Clonetics™ Proliferating Cells are available in a variety of convenient flask and plate formats.
Proliferating cells are delivered Tuesday-Thursday the week after initial plating. Confluency at the time of shipment varies by cell type. A new Clonetics™ Cryovial is used for each new batch using only Clonetics™ Media and Reagents. This procedure ensures viable cells of Benefits of Clonetics™ Proliferating Cells
Save valuable time and materials by using viable cells: No need for expensive thawing and culture expansion. Just order the number of vessels you need.
Proof your results: Validate your cell line experiments with a reliable ready to use primary cell culture
Characteristics of Clonetics™ Proliferating Cells
More than 100 cell types available – normal and diseased
Variety of flasks and plates
Ready-to-assay in plate formats
CoA with donor characteristics
40+ years of experience in primary cells with most publications
