Factor de crecimiento epidérmico EGF de ratón, natural, liofilizado, grado de receptor, 5 viales de 100 µg

Corning Epidermal Growth Factor (EGF), mouse natural (receptor grade),500µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 100µg/container, 5/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

• Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O

Preparation and Storage

• Stable for at least three months at 2° to 8°C (lyophilized) or =one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >98% by SDS-PAGE and pH 9.9 PAGE
• Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma

Source

• Male mouse submaxillary glands

Molecular Weight

• 6.1 kD

Purification

• EGF, mouse natural (receptor grade) - Purified by BioGel P10/DEAE chromatography

Tip

• Recommended concentration: Approximately 5 ng/mL medium depending on cell type