Estándar de referencia KRAS G13D 5_ (formato FFPE) =400 ng_pieza

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild type standard to generate a lower allelic frequencies for limit of detection studies and validation of your pre-analytical phase of the workflow. Horizon’s Base-Seq products are all derived from human cell lines. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more

Technical Data

• DNA Base Change GGC→GAC NCBI Reference Assembly SNP rs112445441
• Cosmic ID COSM532
• Fixation Method 4%
• Formalin Section Size 15µm or 20µm
• Cell Density 3 x 108 cells per block.
• Approx. 3.5 x 105 cells per section
• Expected DNA Yield ≥ 400 ng using Promega Maxwell LEV Plus Extraction kit
• Product information- please check in the Horizon webpage https://www.horizondiscovery.com