Controles Silenciamiento Génico -GIPZ Non-silencing Lentiviral shRNA Control (plasmid)

Disponible
Critical to any gene silencing experiment, RNAi controls enable accurate interpretation for reliable results.
Id. Nav | RHS4347 |
---|---|
Envase | 1 Ud. |
Aplicaciones | Silenciamiento génico |
Categoría de Producto | Controles shRNA |
Subcategoría de Producto | Negativo |
Disponibilidad | Bajo pedido |
Capacidad/Volumen | 1 x glycerol stock |
Especificaciones | All GIPZ shRNA constructs include the TurboGFP reporter gene for convenient tracking of expression and puromycin-resistance for mammalian cell selection. Changes in the mRNA or protein levels in cells treated with a GIPZ negative (non-targeting or non-silencing) shRNA control reflect non-specific responses in cells and should be used as a baseline against which specific knockdown can be measured. A GIPZ positive shRNA control targeting GAPDH or EG5 will confirm whether the construct is successfully delivered into the cell and can activate the RNAi pathway. Controls can also facilitate understanding of transfection efficiency of your cells of interest. Factors affecting transfection efficiency include purity of plasmid DNA, health of transfected cells, inconsistencies in number of cells plated, insufficient mixing of transfection complexes, different cell types, or change in the cell passage number. Determining the transfection efficiency for every RNAi experiment is thus essential to enable valid comparisons across sets of data. Available shRNA Controls Include Non-silencing shRNA construct - The non-silencing shRNA is a negative control for any transfection experiment performed using GIPZ shRNA constructs. When transfected into cells, the endogenous RNAi pathway processes it, but its processed siRNA will not target any mRNA in the mammalian genome. The non-silencing shRNA sequence is cloned into GIPZ lentiviral and TRIPZ lentiviral vectors. This sequence is verified to contain no homology to known mammalian genes. Both glycerol stock and viral particle formats are available. Endogenous positive controls for the GIPZ lentiviral shRNA library include shRNA targeting EG5 (human) and GAPDH (human and mouse). EG5 and GAPDH shRNA containing vectors are available in glycerol stock or viral particle formats. The GAPDH control is available in the TRIPZ vector. Exogenous positive controls are a good choice as positive controls. They control for expression level of your gene in the target cell line and abundance and turnover rate of the target mRNA/protein. Available exogenous positive controls include: β -gal reporter - Monitors transfection efficiencies across wells and under different experimental conditions |
Caracteristicas | Positive and negative GIPZ Lentiviral shRNA Controls for setting the experimental window. |
Marca | HORIZON |
Recomendaciones
-
Controles Silenciamiento Génico -siGENOME siRNA Lamin A_C Control siRNA -Human_Mouse_Rat601,00 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector DNA Damage Response Lentiviral shRNA Pooled Library hCMV-GFP - Human5.600,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus RTF - Ubiquitin Conjugation Subset 2 - custom plates - Human5.106,89 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mEF1a Control UHT2.814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Serine Proteases - SMARTpool - Mouse4.331,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD hCMV-TurboGFP Control Particles - Rat814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
OncoSpan (formato FFPE) estándar de referencia oncogenes240,98 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mCMV-TurboGFP Glycerol 8452,77 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -siGENOME siRNA Library - Ubiquitin Conjugation Subset 3 - SMARTpool - Mouse7.555,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Cell Cycle Regulation Lentiviral shRNA Pooled Library mCMV,GFP - Mouse5.600,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Expresión Génica -Yeast Parental Strain BY4730 (glycerol stock)114,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control mCMV-None UHT 32.814,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -Accell Cyclophilin B siRNA - Rat190,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenc. Génico SMARTvector Inducible Ubiquitin Conjugation Lentiviral shRNA Pooled Library mCMV-RFP, Human6.721,24 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus siRNA Set of 4 - Mouse1.436,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -shMIMIC Lentiviral microRNA Pooled Library - Human-Mouse Conserved hEF1a-RFP3.947,71 € sin ivaPara ver tus precios y comprar inicia sesión