Highlights
• Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in human or mouse genomes
• Choose an individual or pooled crRNA control to match your experimental crRNA reagent
Caracteristicas
Edit-R CRISPRa crRNA Non-targeting Controls are designed and recommended for use as negative controls for transcriptional activation experiments using Edit-R CRISPRa crRNA individuals and pools. These non-targeting controls will hybridize with tracrRNA and engage the dCas9-VPR complex, but will not target any PAM-adjacent sites in the human or mouse genome. Any observed alteration in gene expression levels or viability in cells treated with these controls can be used as a baseline response of the cells to the dCas9-VPR complex for comparison to those treated with target-specific CRISPRa crRNAs and pools.