Control positivo, PepMV, 1 tubo_2,5mL

PepMV (6), a potexvirus, was first reported in pepino (Solanum muricatum) in Peru (2). In Europe, first outbreaks of PepMV in greenhouse tomato have been reported 1999/2000 in the Netherlands, Germany, France, the UK and Spain, subsequently in many European countries as well as in Canada, USA and China. Other solanaceous plants such as potatoes could be infected by artificial inoculation, but so far no PepMV problem has been observed in that crop. Different PepMV genotypes have been recognized (3, 5). Transmission occurs by contact, e.g. by clothes, contaminated tools, fruits and other plant parts, and at very low rate, by seed. PepMV is on the EPPO ‘Alert List’ and is a regulated pathogen on tomato seeds in the EU. Containment of disease is achieved by sanitary measures, including seed disinfection (e.g. commercial bleach solution, 4) and seed testing. DAS-ELISA (1) is one of the established methods for PepMV detection in plants and seeds.