CloneticsAsthmatic Airway Cells include lung fibroblasts, bronchial epithelial and bronchial smooth muscle cells. Lung fibroblasts are isolated from adult lung tissue, bronchial epithelial cells are isolated from epithelial lining of airways above bifurcation of the lungs, bronchial smooth muscle cells are isolated from the major bronchia.
Asthma is an inflammatory disorder of the airways, which causes attacks of wheezing, shortness of breath, chest tightness, and coughing in patients. Degrees of severity are unique to individual patients as are responses to drug treatments. Adhesion of eosinophils to bronchial epithelial cells, and the interaction of airway smooth muscle and sub-epithelial fibroblasts play a critical role in asthma pathology. During an asthma attack bronchiole smooth muscle tissue can constrict and decrease the flow of air in the airways. The amount of air flow can further be decreased by inflammation or excess mucus secretion.Cryopreserved CloneticsDiseased Lung fibroblasts cells stain negative for von Willebrand factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin.. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended medium: FGM-2 BulletKit
Cryopreserved CloneticsDiseased Bronchial Epithelial cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended medium: BEGMBulletKit
Cryopreserved CloneticsDiseased Bronchial Smooth Muscle cells stain positive for alpha smooth muscle actin and negative for von Willebrand factor VIII. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended medium: SmGM-2 BulletKit
In addition to cryopreserved cell ampules Lonza also offers viable cells: Clonetics™ Proliferating Cells are available in a variety of convenient flask and plate formats.
Proliferating cells are delivered Tuesday-Thursday the week after initial plating. Confluency at the time of shipment varies by cell type. A new Clonetics™ Cryovial is used for each new batch using only Clonetics™ Media and Reagents. This procedure ensures viable cells of Benefits of Clonetics™ Proliferating Cells
Save valuable time and materials by using viable cells: No need for expensive thawing and culture expansion. Just order the number of vessels you need.
Proof your results: Validate your cell line experiments with a reliable ready to use primary cell culture
Characteristics of Clonetics™ Proliferating Cells
More than 100 cell types available – normal and diseased
Variety of flasks and plates
Ready-to-assay in plate formats
CoA with donor characteristics
40+ years of experience in primary cells with most publications
