Able K Electro-Comp Cells 5 x 0.1 ml
Disponible
ABLE K Competent Cells for toxic clones enhance the probability of cloning DNA encoding toxic proteins. By reducing the copy number of ColE1-derived plasmids, the ABLE K strain decreases the levels of cloned gene products and enhances the probability of propagating a toxic clone.
ABLE K Competent Cells provide a viable alternative to commonly used cloning vectors with high copy numbers. Positive clones observed on initial screening of lambda libraries can be excised or re-cloned into any convenient vector and can be introduced into ABLE K strains.
ABLE K Competent Cells provide a viable alternative to commonly used cloning vectors with high copy numbers. Positive clones observed on initial screening of lambda libraries can be excised or re-cloned into any convenient vector and can be introduced into ABLE K strains.
For Research Use Only. Not for use in diagnostic procedures.
Features
- Reduces the copy number of ColE1-derived plasmids (such as pUC and pBluescript® plasmids) 10-fold
- Decreases level of cloned protein product to increase cell viability
- Transformation efficiency of 5 x 106 - 2 x 107 transformants/µg
- Also available in an electroporation-competent version
Specifications
- Blue-White Screening Available: Yes
- Cell Strain: ABLE K
- Cell Type - Production Method: Electroportable Cells
- Competent Cell Application:
- Cloning Toxic DNA through modulation of plasmid copy number
- Difficult Cloning Type: Toxic Clone
- Generates Unmethylated DNA: No
- Resistance: Kanamycin, Tetracycline
- Transformation Efficiency – Qualitative: High
- Transformation Efficiency – Quantitative: 1 x 1010
- Volume : 5 x 0.1 ml
Id. Nav | 200162 |
---|---|
Envase | 5 x 0,1 ml |
Aplicaciones | Clonaje |
Categoría de Producto | Células competentes |
Disponibilidad | Bajo pedido |
Marca | AGILENT |
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