Kit de RT-qPCR de dos pasos para amplificación de ARN, 100x20µl rxns, 4x0,1ml mix, RTasa 1x0,1ml

High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. 

The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. 

Features

• Unbiased representation of 5’ and 3’ mRNA transcript ends
• Sensitive detection of low copy number transcripts
• High cDNA yields from as little as 4pg total RNA
• Reduced RNase H activity 
• Simple 2 tube system
• 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
• 20x thermostable reverse transcriptase blended with RNase inhibitor